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PACECS: Processes and Approaches of Coastal Ecosystem Carbon Sequestration/ MEMCS: Mechanisms and Environmental Effects of Microbes on Carbon Sequestration
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Carbon dioxide sequestration by metabolically diverse marine bacteria for production of bioplastics
P-SPS2-12-S Peiwen Zhan* , State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Yu Han, State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Xiaofeng Chen, State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Dan Lin, State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Nianzhi Jiao, Kai Tang, State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Presenter Email: zhan-wen@foxmail.com |
Polyhydroxyalkanoates (PHA) can be synthesized by various microorganisms as intracellular materials for the storage of carbon and energy. The present work involved investigation of a previously reported carbon concentrating Roseobacter clade bacterial strain Thiobacimonas profunda JLT2016 for production of PHA under different metabolisms. Nile red fluorescence proved the presence of lipidic granules within the bacterial cells. Transmission electron microscopy was also done. PHA produced by JLT2016 was identified as PHB according to the characteristic peaks detected in FT-IR spectrum and further confirmed by 1H and 13C NMR spectrums. T. profunda JLT2016 was incubated under heterotrophic culture, chemolithoheterotrophic culture and mixotrophic culture with glucose, sodium sulphide and sodium bicarbonate served as substrates of energy source. The highest PHA production was obtained under chemolithoheterotrophic culture, in which PHB yields reached 60% of cell dry weight (CDW) and CDW reached 2.872 g/L. The experiments showed feasibility in using waste as raw materials to cut down the production costs through the application of metabolically diverse marine bacteria.
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