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PACECS: Processes and Approaches of Coastal Ecosystem Carbon Sequestration/ MEMCS: Mechanisms and Environmental Effects of Microbes on Carbon Sequestration
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The patterns of DOM transformation and utilization by heterotrophic bacteria in phytoplankton bloom revealed by proteomic and metabolomic analyses.
P-SPS2-03-S
Yu Han* , State Laboratory of Marine Environmental Science, Xiamen University, China.
Kai Tang, State Laboratory of Marine Environmental Science, Xiamen University, China.
Chen He, China University of Petroleum (Beijing), China.
Quan Shi, China University of Petroleum (Beijing), China.
Ruanhong Cai,
Nianzhi Jiao, State Laboratory of Marine Environmental Science, Xiamen University, China.
Presenter Email: yuhan1019@foxmail.com |
| Nutrients pollution and eutrophication have become the main threats to the ecological environment in coastal waters, which have led to algal blooms outbreak frequently in many gulf and estuary. Based on the study of Akashiwo sanguinea, gymnodinium bloom in Xiamen coastal ocean, analysis of dominant bacterial community including Flavobacteriales, Rosebacter clade, Oceanospirillales, explaining the micro process of utilization and transformation of dissolved organic matter in algal bloom by heterotrophic bacteria utilizing proteomics and high-throughput metabolomics (FT-ICR-MS). In terms of proteomics, we found Flavobacteriales expressed nearly 76% of the annotation protein is significantly higher than Roseobacter clade (49%) and Oceanospirillales (56%), of which Flavobacteriales rich in amino acid metabolism related protein expression, and Roseobacter clade and Oceanospirillales prefer to ABC transporter, membrane proteins and two-component system related protein expression. The metabolites of utilization was organic nitrogen (e.g., peptide) on Flavobacteriales, organic sulfur and other substances (such as DMSP) on Roseobacter clade. Therefore, the dissolved organic matter may control the succession of bacterial community in algal bloom, and the bacteria can participate in the circulation of the elements through the transformation of the dissolved organic matter. |
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