Denitrification and anaerobic ammonium oxidation (anammox) are two dominant processes for reactive nitrogen removal. In the context of global warming, temperature may serve as the primary forcing function to tune the microbial metabolism thus the community distribution, yet, how the community change and associated nitrogen removal pathways respond to warming remains a knowledge gap in prediction of nitrogen cycling and potential climate feedbacks. By using ¹⁵N isotope pairing technique, we measured the potential rates of sedimentary denitrification, anammox, and associated N₂O production at a temperature gradient ranging from 1-35℃ in various aquatic systems, including estuary, aquaculture pond, tidal flat and freshwater creek. The optima temperature and apparent activation energy for the denitrification were systematically and significantly higher than those for anammox. The N₂O production rate was stimulated by temperature increase. While the N₂O/N₂ of denitrification exhibited a bimodal structure with higher values at both 1-15℃ and 30-35℃. Combined our experimental results with global compilation of the existing data on in situ sedimentary denitrification, anammox, N₂O release and N₂O/N₂ temperature dependence in various aquatic systems, we inferred that (1) denitrifying communities adapt to a higher and a wider temperature range, while the anammox bacteria were relatively cold-adapted; (2) N₂O reductase was more easily to be suppressed than other enzymes participating the N₂O production outside the habitable temperature.