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Physics of estuaries and coastal seas
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Characterization and expression profiling of trypsin in Phaeodactylum tricornutum
P-P1-15
Yanchun You* , Xiamen University
Senjie Lin, Xiamen University
Presenter Email: fjyhy@163.com |
| Trypsin (EC 3.4.21.4) is a serine protease that cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine, and can be found in many organisms. Trypsin is multifunctional enzyme and has been found involved in many important physiological processes in animal, such as food digestion, hemostasis, immune defense response and nerve response. In addition, it has been used widely in various biotechnological processes. However, the function of marine phytoplankton trypsin is still unknown. Interestingly, the diatom trypsin genes showed high expression in our previous metatranscriptome data of marine harmful algae. Hence, trypsin may play an important role in the growth of marine phytoplankton. In this study, the typical marine diatom Phaeodactylum tricornutum was selected as model species to explore the function of trypsin in marine phytoplankton. The public published P. tricornutum genome data provide an opportunity to investigate the structure, function, and evolution of trypsin on a genome-wide scale. Ten putative trypsin genes have been identified in P. tricornutum by searching the P. tricornutum genome, and named according to the order of position on the genome. Phylogenetic analyses on the trypsin amino acid sequences reveal that the ten trypsin could be divided into two main subclasses. Furthermore, expression of all the trypsin genes in different life stages and culture conditions was detected by qRT-PCR. PtTrp1 showed no significant expression between different culture conditions. PtTrp2 and PtTrp6 showed high constitutive expression in various life stages. PtTrp2, PtTrp6, PtTrp9 and PtTrp6 exhibited higher expression in P-deficiency. The diversified features and expression patterns of the trypsin are inferred to be associated with the alga growth. Our findings provide a base for functional research on phytoplankton trypsin, a better understanding of the outbreak mechanism of harmful algal blooms, and strategies for monitoring harmful algal blooms. |
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