| |
| |
|
|
|
|
| |
 |
The role of trace metals in controlling structure and function of microbial communities in contemporary oceans
|
| |
|
|
Functions of three predicted genes associated with biosilicification in the diatom Thalassiosira pseudonana
P-C3-02-S
Peng-Yu Ji* , School of Life Sciences, Xiamen University, Xiamen 361102, China
Shan-Shan Zhuang, School of Life Sciences, Xiamen University, Xiamen 361102, China
Lu Huang, School of Life Sciences, Xiamen University, Xiamen 361102, China
Chang-Ping Chen, School of Life Sciences, Xiamen University, Xiamen 361102, China
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China
Ya-Hui Gao, School of Life Sciences, Xiamen University, Xiamen 361102, China
State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361102, China
Jun-Rong Liang*, School of Life Sciences, Xiamen University, Xiamen 361102, China
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China
Corresponding author: sunljr@xmu.edu.cn
Presenter Email: j1yue1993@gmai.com |
| Marine diatoms are one of the most successful groups of algae and their photosynthesis in the oceans is known to be an extremely important component of global element cycles. Despite their significant roles in global marine carbon fixation, diatoms are also a major group of siliceous organisms, contributing to the global silicon cycle. Up to now, several proteins or component associated with silica cell wall formation, such as silaffin, cingulin, have been discovered. But the function of a number of proteins associated with silicon metabolism still remains unknown. In the present study, 19 candidate genes without function annotation (named as predicted genes 1-19) were detected to potentially associated with cell wall synthesis based on their genes expressions patterns in transcriptomics and structural characteristics (KXXK domain, RXL domain, signal peptide). Real-time PCR results have verified that genes expression models of these predicted genes 1-19 have a significant relationship with silicon metabolism during synchronization process. Transgenic vectors of P3, P13, P19 were made to verify the roles of these genes in the formation of siliceous cell walls. This study improves our understanding of the biosilicification process and silicon metabolism and provides a brand new view of the peculiarity of diatoms in marine ecosystem.
This work was supported by the National Natural Science Foundation of China (Grant No. 41576138 and 41276130).
keywords: diatom, Thalassiosira pseudonana, biosilicification, Si-related genes
|
|
|
|
|
|
|
|
|