| |
| |
|
|
|
|
| |
 |
The role of trace metals in controlling structure and function of microbial communities in contemporary oceans
|
| |
|
|
Transcriptome analyses of silicon metabolism in the resting cells of marine diatom Thalassiosira pseudonana
P-C3-06-S
Shan-Shan Zhuang* , School of Life Sciences, Xiamen University, Xiamen 361102, China
Qian-Qian Huang, School of Life Sciences, Xiamen University, Xiamen 361102, China
Peng-Yu Ji, School of Life Sciences, Xiamen University, Xiamen 361102, China
Chang-Ping Chen, School of Life Sciences, Xiamen University, Xiamen 361102, China
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China
Lin Sun, State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361102, China
Ya-Hui Gao, School of Life Sciences, Xiamen University, Xiamen 361102, China
State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361102, China
Jun-Rong Liang*, School of Life Sciences, Xiamen University, Xiamen 361102, China
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China
Corresponding author: sunljr@xmu.edu.cn
Presenter Email: zss133@foxmail.com |
| Diatom resting stage (including resting cell and resting spore) must be considered as an effective strategy for maintaining diatom populations in marine ecosystems under adverse conditions. One of outstanding characteristics of resting stage cells is producing more heavily silicified frustules, which increasing the sinking rates of the cells to improve the export of the carbon and silicon into the deeper ocean as biological pump. However, the mechanism of biosilicification during resting stage cell formation in diatoms is still unknown. Here, the resting cells of the modern diatom Thalassiosira pseudonana were induced under dark and 4 degree centigrade for three months. Cellular response and genes involved in biosilicification during resting cells formation were investigated using transcriptome analysis. A total of 11882 unique genes were identified in six time points (weeks 0, 4, 6, 8, 10, 12) and 4840genes with significant expression levels were selected for further analysis. Especially, the genes related to girdle band (Cingulin W2) and valve formation (Silaffin 4) kept up-regulation in the process of resting cells formation, indicating their roles in heavy silicification. The function analysis in the genes that co-varying in expression with Cingulin W2 and sillafin 4 has revealed the cellular responses associated with silicon metabolism and identified some new specific proteins containing a unique domain. Our findings have provided a new insight into silicon metabolism associated with resting cells formation, which will enhance our understanding of the role of diatom resting cells in biogeochemical cycles of silicon and carbon. This work was supported by the National Natural Science Foundation of China (Grant No. 41576138 and 41276130). Keywords: diatom, Thalassiosira pseudonana, resting cells, biosilicification, silica-forming genes, biogeochemical cycles of silicon and carbon |
|
|
|
|
|
|
|
|