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The role of trace metals in controlling structure and function of microbial communities in contemporary oceans
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The interrelationship between cell size, cellular cadmium and cadmium-carbonic anhydrase in marine diatoms
Tuesday 8th @ 1130-1150, Conference Room 1
Weiying Li* , State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
Haizheng Hong, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
Dalin Shi, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
Presenter Email: lwy@stu.xmu.edu.cn |
| The discovery of cadmium-carbonic anhydrase (CDCA), the only known biological function of cadmium (Cd), provides a link between the biogeochemical cycles of carbon and Cd. Previous studies indicate that cellular Cd concentration of phytoplankton likely correlates positively with their size. However, it remains unclear if such a relationship involves CO2 acquisition facilitated by CDCA. In this study, we investigated the interrelationship between cell size, cellular Cd concentration, and CDCA in three centric marine diatom species (i.e., Thalassiosira pseudonana, Thalassiosira weissflogii and Ditylum brightwellii) spanning a range of cell sizes of 50 to 6000 um3. Larger diatoms did have higher cellular Cd:C ratios at a given growth rate, and for a similar increase in growth rate as a result of Cd addition, CA activity increased by 21%, 56% and 88%, respectively in T. pseudonana, T. wesfloggii and D. brightwellii. In addition, the gene transcription and protein expression of CDCA were found to increase along with CA activity upon Cd addition. Taken together, our study showed that larger diatom cells would need more Cd to enhance CA activity to support inorganic carbon acquisition for growth. |
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