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Harmful algal blooms: mechanisms, monitoring, and prevention in a rapidly changing world
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Metatranscriptome analysis reveals environmental and diel regulation of a Heterosigma akashiwo (Raphidophyceae) bloom
P-B1-05-S
Nanjing Ji* , State Key Laboratory of Marine Environmental Science, Xiamen University
Lingxiao Lin, State Key Laboratory of Marine Environmental Science, Xiamen University
Ling Li, State Key Laboratory of Marine Environmental Science, Xiamen University
Liying Yu, State Key Laboratory of Marine Environmental Science, Xiamen University
Yaqun Zhang, State Key Laboratory of Marine Environmental Science, Xiamen University
Hao Luo, State Key Laboratory of Marine Environmental Science, Xiamen University
Meizhen Li, State Key Laboratory of Marine Environmental Science, Xiamen University
Xinguo Shi, State Key Laboratory of Marine Environmental Science, Xiamen University
Da-Zhi Wang, State Key Laboratory of Marine Environmental Science, Xiamen University
Senjie Lin, State Key Laboratory of Marine Environmental Science, Xiamen University
Presenter Email: jinanjing@126.com |
| Despite numerous laboratory studies on physiologies of harmful algal bloom (HAB) species, physiologies of these algae during a natural bloom are understudied. Here, we investigated a bloom of the raphidophyte Heterosigma akashiwo in the East China Sea in 2014 using metabarcode (18S rDNA) and metatranscriptome sequencing. Based on 18S rDNA analyses, the phytoplankton community shifted from high diversity in the pre-bloom stage to H. akashiwo predominance during the bloom. A sharp decrease in ambient dissolved inorganic phosphate and strong up-regulation of phosphate and dissolved organic phosphorus (DOP) uptake genes, including the rarely documented (ppGpp)ase, in H. akashiwo from pre-bloom to bloom was indicative of rapid phosphorus uptake and efficient utilization of DOP that might be a driver of the H. akashiwo bloom. Furthermore, observed up-regulated expression of mixotrophy-related genes suggests potential contribution of mixotrophy to the bloom. Accelerating photosynthetic carbon fixation was also implied by the up-regulation of carbonic anhydrase genes during the bloom. Notably, we also observed a strong morning-to-afternoon shift in the expression of many genes. Our findings provide insights into metabolic processes likely important for H. akashiwo bloom formation, and suggest the need to consider timing of sampling in field studies on this alga. |
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