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Harmful algal blooms: mechanisms, monitoring, and prevention in a rapidly changing world
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Rare Bacteria in Seawater
Are Dominant of Bloom-forming Dinoflagellate Noctiluca Scintillans Associated Bacterial Assemblage
P-B1-13
Xiaomin Xia* , South China Sea Institute of Oceanology, CAS
Suki Leung, The Hong Kong University of Science and Technology
Hongbin Liu, The Hong Kong University of Science and Technology
Presenter Email: xxia@connect.ust.hk |
| Noctiluca scintillans, a bloom-forming dinoflagellate, is widely distributed in the global ocean. Endocytic bacteria of zooplanktons have been widely reported and proved to be essential for survival and growth of zooplanktons. However, few study investigated diversity of endocytic bacteria of Noctiluca scintillans. In this study, we applied 454 pyrosequencing and metagenomic sequencing to investigate the diversity,characteristics and function of endocytic bacteria in Noctiluca. Endocytic bacteria were discovered in both field and lab Noctiluca cells, with higher stability community composition in the lab Noctiluca cells while greater variation in the field Noctiluca cells collected at different times. Bacterial communities of lab Noctiluca cells were mainly dominated by Rhodobacterales, while dominant species varied with sampling times in the field Noctiluca samples. Most of the endocytic bacteria were found in the environment where Noctiluca cells lived in, with low relative abundance. Cultivating the field Noctiluca cells with mono-specific food source shifted the bacterial communities in Noctiluca cells. Ampicillin caused death of Noctiluca cells, suggesting a critical role of endocytic bacteria to survival of Noctiluca. Metagenomic analysis shows that prop and ABCB-BAC were the two most abundant genes involved in the membrane transport.Our data suggested that bacteria widely distribute in Noctiluca cells, and endocytic bacterial community composition is related to the environmental condition that Noctiluca cells live in and is important to the survival of Noctiluca. |
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