Program

Abstract Fourier Transform Spectroscopy was employed for determination of biomacromolecular contents (mainly refers to carbonhydrates, lipids and proteins) in Phaeodactylum tricornutumof different stage cultured under 10oC, 15oC, 20oC and 25oC and different light intensity(1000lx, 2000lx, 3000lx and 4000lx). The results showed that different tendencies appeared at different temperatures. The trend of biomacromolecular contents at 15oC and 25oC were samilar, which accumulated with the extension of incubation time in platform stage. While at 20oC, carbonhydrates and proteins contents grew to the peak at early stage of platform, which was different from tendency of lipids. And the content of lipids at 20oC maximized at metaphase of platform, lagging behind carbonhydrates and proteins. Transmission electron microscopy images also proved the highest lipid content at 15 oC. The highest lipid, protein and carbohydrate content were obtained under 2000lx light intensity, and decreased with  light intensity increasing. Fourier Transform Spectroscopy method can detect relative contents of multiple biomacromolecular of P. tricornutum using a little sample, which provides a new way for further research on metabolic mechanism.