|  |
Program |
 |
| |
 |
Special Session 1: Ecosystem under multiple stressors |
| |
|
|
Function analysis of rhodopsin in dinoflagellate
SS1-11-S
Minglei Ma* , Marine Ecogenomics Laboratory
Presenter Email: 18850583965@163.com
|
| Rhodopsins are now found in all domains of life. Based on amino acid sequences, rhodopsin proteins are classified into two large groups: type I, found in animal and type II found in microbial including Bacteria, Archaea, and Eukarya. The latter type of rhodopsin has been reported to convert light into ATP directly through light-driven proton pump in the ocean. Rhodopsin genes similar to that encoding proton-pumping rhodopsin in bacteria have been found in dinoflagellates. However, the function of dinoflagellate rhodopsin is still poorly understood. In order to address the gap of knowledge, we constructed expression plasmid for Prorocentrum donghaisense and Alexandrium fundyence rhodopsin genes to transfect 293HEK cells. Total RNA was extracted from the stable transformed cells and RT-PCR performed. The result suggested that both of the P.donghaiense and Alexandrium rhodopsin have been transfected to 293T successfully. We will focus on studying functions of the two rhodopsin genes. One is to test the expression of rhodopsin protein by Western Blot. And then we will analyze the absorption spectra of this protein. In addition, we will examine cell growth to determine if cell multiplication can benefit from the rhodopsin under illumination. |
|
|
|
|