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General Session 3: Biological oceanography & global change |
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Metaproteomics reveals metabolic activities between the blooming and non-blooming samples of phytoplankton collected at the coastal East China Sea
GS3-70
Hao Zhang* , State Key Laboratory of Marine Environmental Science, Xiamen University
Shufeng Zhang, State Key Laboratory of Marine Environmental Science, Xiamen University
Dongxu Li, College of the Environment and Ecology, Xiamen University
Lingfen Kong, College of the Environment and Ecology, Xiamen University
Xiaohuang Chen, College of the Environment and Ecology, Xiamen University
Lin Lin, State Key Laboratory of Marine Environmental Science, Xiamen University
Dazhi Wang, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University
Presenter Email: mrzhang0539@163.com
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| Although much work has been devoted to the target bloom species, knowledge about the metabolic activities of all phytoplankton communities are poorly understood. In this study, we used metaproteomics to investigate the metabolic activities of microalgae between the blooming and surrounding non-blooming samples of phytoplankton collected from the coastal East China Sea. After searching the combined database, 3912 and 2762 high confidence proteins were identified from the two samples. The Dinophyta species took the biggest proportion within the two samples, following by Bacillariophyta, Cyanophyta, Ochrophyta and Haptophyta populations. For Dinophyta, the biological processes of translation, transcription, protein turnover and modification, and photosynthesis were higher expressed in blooming sample to non-blooming sample, reflecting active protein synthesis and higher demand of light energy. For non-blooming species, the proteins involved in energy production and conversion, translation and photosynthesis were dominantly identified. The varying expression of light harvest and pigment proteins, and the higher abundance of high affinity organic compound binding ABC transporters of non-blooming species revealed their specific and preferential light utilization and switched into organic compound utilization mechanisms. In summary, our analyses revealed concomitant microalgae interactions of numerous metabolic activities in the coastal area that are central to light, carbon, and nutrients utilization in the coastal marine environment. |
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