Program
 
General Session 3: Biological oceanography & global change
 
 
 
Poster
CO2 exhaustion induces the expression of C4 cycle of a marine dinoflagellate Prorocentrum donghaiense
GS3-36-S
Hao Zhang, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
Tianqi Liu* , State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
Lin Lin, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
Dazhi Wang, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
Presenter Email: 925422991@qq.com
Dinoflagellate blooms are one of the worldwide economic and ecological concerns. Many efforts have been devoted to nutrients regulation on dinoflagellate blooms, however, understanding of carbon oxide assimilation has been lacking. Here, we used transcriptome, metaproteomics and isotope ratio MS techniques to prove the existence of C4 cycle of Prorocentrum donghaiense, and the C4 cycle was induced to highly express within the blooming cells in marine environment. In laboratory, higher cell density and Fv/Fm were observed in CO2 sufficient when compare to CO2 exhaustion condition. The proportion of 13C of particle organic carbon were increased as the CO2 exhaustion aggravated in laboratory, and same results was observed in blooming cells of P. donghaiense in marine environments. Moreover, nearly all genes involved in C4 cycle were cloned in pure P. donghaiense cells and higher abundances were monitored in CO2 exhaustion conditions. In summary, we firstly proved the existence of C4 cycle in P. donghaiense and its expression showed negative correlation with dissolved inorganic carbon concentration. This study highlighted the viral adaptive mechanisms of CO2 assimilation during blooming conditions, and also deepened our knowledge on maintenance mechanisms of dinoflagellate blooms in marine environment.