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General Session 4: Marine environment, ecosystem & sustainability |
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Transcriptomic analysis reveals novel molecular mechanisms involved in phosphorus acclimation in a marine dinoflagellate prorocentrum donghaiense
GS4-44-S
Shufeng Zhang* , State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Chunjuan Yuan, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Ying Chen, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Xiaohuang Chen, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Dongxu Li, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Jiuling Liu, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Lin Lin, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Dazhi Wang, State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Presenter Email: zhangshusss@163.com
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Dinoflagellates are one of the major contributors to primary production in the ocean and major causative agents of harmful algal blooms in the coastal waters. Phosphorus (P) is an essential macronutrient limiting marine dinoflagellate growth and productivity. However, the molecular mechanisms involved in P acclimation are poorly understood in marine dinoflagellates. Here, we compared the transcriptomes of a marine dinoflagellate Prorocentrum donghaiense grown in inorganic P-replete, P-deplete, and inorganic- and organic P-resupplied conditions using RNA-Seq and characterized differentially expressed genes. Transcripts of 27,434 genes altered significantly in P-deplete cells. Genes encoding low- and high-affinity phosphate transporters were down-regulated while genes participating in organic P utilization, nucleotide metabolism, photosynthesis, glycolysis and cell cycle were up-regulated. Remarkably, several genes involved in regulating photoperiod and circadian rhythm, such as flavin-binding kelch repeat F-box protein 1, were firstly identified in marine dinoflagellates and were down-regulated in P-deplete cells. Our results indicated that, in contrast with other algal species, P. donghaiense possessed a specific ability to utilize organic P, and ambient P depletion disturbed the circadian rhythm of P. donghaiense which subsequently triggered the response mechanisms to ambient P change.
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