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Program |
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General Session 3: Biological oceanography & global change |
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The infection of demethylation of genomic DNA to the Symbiodinium Kawagutii
GS3-08-S
Yang Feng* , Marine Ecogenomics Laboratory
Presenter Email: 22320161151348@stu.xmu.edu.cn
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From the genome sequencing of Symbiodinium.Kawagutii, the researchers found that the methylation of genomic DNA in Symbiodinium.Kawagutii is common, and a lot of methyltransfers gene copies, however, the function of this broad DNA methylation in the species are still unknown. In order to identify the function of methylation of DNA, we used demethylation drugs 5กฏ-AZA-2กฏ-deoxycytidine (5กฏ-AZA-CDR) to treat the Symbiodinium.Kawagutii in the laboratory and try to understand how the demethylation of genome affect the growth curves, cell size, particle characteristic and cell cycle of Symbiodinium.Kawagutii as well as the changes of nutrient uptake. There are four groups of cell culture medium which add 5กฏ-AZA-CDR with different concentration. The results shows significant divergence in growth between the four groups, but cell size, particle characteristic and photosynthetic efficiency do not shows an obviously difference. The results indicated that 5กฏ-AZA-CDR would affect the cell growth, which might caused by the demethylation of genomic DNA. Basis on these results, we would design the further experiment to pursue the following goals: 1) whether or not the demethylation of genomic DNA will affect the cell cycle. 2) whether or not it will affect the gene expression. 3) whether or not it will affect the nutrient uptake.
Keywords: symbiodinium.Kawagutii, genome demethylation, cell cycle, gene expression, nutrient uptake
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